Acute inhibition of Na/H exchanger NHE-3 by cAMP: role of protein kinase a and NHE-3 phosphoserines 552 and 605
H Zhao, MR Wiederkehr, L Fan, RL Collazo… - Journal of Biological …, 1999 - ASBMB
Regulation of the renal Na/H exchanger NHE-3 by protein kinase A (PKA) is a key
intermediate step in the hormonal regulation of acid-base and salt balance. We studied the
role of NHE-3 phosphorylation in this process in NHE-deficient AP-1 cells transfected with
NHE-3 and in OKP cells expressing native NHE-3. A dominant-negative PKA-regulatory
subunit completely abolished the effect of cAMP on NHE-3 activity demonstrating a role of
PKA in the functional regulation of NHE-3 by cAMP. NHE-3 isolated from cAMP-treated cells …
intermediate step in the hormonal regulation of acid-base and salt balance. We studied the
role of NHE-3 phosphorylation in this process in NHE-deficient AP-1 cells transfected with
NHE-3 and in OKP cells expressing native NHE-3. A dominant-negative PKA-regulatory
subunit completely abolished the effect of cAMP on NHE-3 activity demonstrating a role of
PKA in the functional regulation of NHE-3 by cAMP. NHE-3 isolated from cAMP-treated cells …
