[HTML][HTML] A novel glycosulfopeptide binds to P-selectin and inhibits leukocyte adhesion to P-selectin
A Leppänen, P Mehta, YB Ouyang, T Ju, J Helin… - Journal of Biological …, 1999 - ASBMB
P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric membrane mucin on leukocytes that
binds selectins. The molecular features of PSGL-1 that determine this high affinity binding
are unclear. Here we demonstrate the in vitro synthesis of a novel glycosulfopeptide (GSP-6)
modeled after the extreme N terminus of PSGL-1, which has been predicted to be important
for P-selectin binding. GSP-6 contains three tyrosine sulfate (TyrSO 3) residues and a
monosialylated, core 2-based O-glycan with a sialyl Lewis x (C2-O-sLe x) motif at a specific …
binds selectins. The molecular features of PSGL-1 that determine this high affinity binding
are unclear. Here we demonstrate the in vitro synthesis of a novel glycosulfopeptide (GSP-6)
modeled after the extreme N terminus of PSGL-1, which has been predicted to be important
for P-selectin binding. GSP-6 contains three tyrosine sulfate (TyrSO 3) residues and a
monosialylated, core 2-based O-glycan with a sialyl Lewis x (C2-O-sLe x) motif at a specific …