[HTML][HTML] Binding of High Density Lipoprotein (HDL) and discoidal reconstituted HDL to the HDL receptor scavenger receptor Class B Type I*: effect of lipid association …
KN Liadaki, T Liu, S Xu, BY Ishida… - Journal of Biological …, 2000 - ASBMB
The binding of apoA-I-containing ligands to the HDL receptor scavenger receptor class B
type I (SR-BI) was characterized using two different assays. The first employed conventional
binding or competition assays with 125 I-labeled ligands. The second is a new
nonradioactive ligand binding assay, in which the receptor-associated ligand is detected by
quantitative immunoblotting (" immunoreceptor assay"). Using both methods, we observed
that theK d value for spherical HDL (density= 1.1–1.13 g/ml) was∼ 16 μg of protein/ml, while …
type I (SR-BI) was characterized using two different assays. The first employed conventional
binding or competition assays with 125 I-labeled ligands. The second is a new
nonradioactive ligand binding assay, in which the receptor-associated ligand is detected by
quantitative immunoblotting (" immunoreceptor assay"). Using both methods, we observed
that theK d value for spherical HDL (density= 1.1–1.13 g/ml) was∼ 16 μg of protein/ml, while …
