ABCA1, the ATP-binding cassette protein mutated in Tangier disease, mediates the efflux of
excess cellular sterol to apoA-I and thereby the formation of high density lipoprotein. The
intracellular localization and trafficking of ABCA1 was examined in stably and transiently
transfected HeLa cells expressing a functional human ABCA1-green fluorescent protein
(GFP) fusion protein. The fluorescent chimeric ABCA1 transporter was found to reside on the
cell surface and on intracellular vesicles that include a novel subset of early endosomes, as …