Tissue plasminogen activator-induced secretion of type-1 plasminogen activator inhibitor in cultured human fibroblasts
TM Reilly, G Greenplate, P Timmermans - Thrombosis research, 1989 - Elsevier
Tissue plasminogen activator (t-PA) regulation of type-1 plasminogen activator inhibitor (PAI-
1) secretion by WI-38 human fibroblasts was investigated utilizing a specific ELISA to
measure PAI-1 antigen levels. Incubation of confluent fibroblasts with t-PA in serum-free
medium resulted in a concentration and time-dependent increase in the secretion of PAI-1,
with the maximum response (a two-fold increase) observed following a 4 hour incubation
with 0.1 μM t-PA. This t-PA effect was reduced by cycloheximide, suggesting that the …
1) secretion by WI-38 human fibroblasts was investigated utilizing a specific ELISA to
measure PAI-1 antigen levels. Incubation of confluent fibroblasts with t-PA in serum-free
medium resulted in a concentration and time-dependent increase in the secretion of PAI-1,
with the maximum response (a two-fold increase) observed following a 4 hour incubation
with 0.1 μM t-PA. This t-PA effect was reduced by cycloheximide, suggesting that the …