Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide
BT Kren, A Cole-Strauss, EB Kmiec, CJ Steer - Hepatology, 1997 - journals.lww.com
Although a variety of methods has been devised for modification of hepatic genes, none has
been effective for long-term correction of genetic disorders. In this study, we employed a
recently described novel experimental strategy for site-directed nucleotide exchange in
genomic DNA of HuH-7 human hepatoma cells. A chimeric 2′-O-methylated-RNA/DNA
oligonucleotide containing sequences complementary to 25 bases of the alkaline
phosphatase gene was constructed as a duplex containing a G to A substitution at …
been effective for long-term correction of genetic disorders. In this study, we employed a
recently described novel experimental strategy for site-directed nucleotide exchange in
genomic DNA of HuH-7 human hepatoma cells. A chimeric 2′-O-methylated-RNA/DNA
oligonucleotide containing sequences complementary to 25 bases of the alkaline
phosphatase gene was constructed as a duplex containing a G to A substitution at …
