Effects of pH and free Mg2+ on the Keq of the creatine kinase reaction and other phosphate hydrolyses and phosphate transfer reactions.
JW Lawson, RL Veech - Journal of Biological Chemistry, 1979 - ASBMB
The observed equilibrium constants (Kobs) of the creatine kinase (EC 2.7. 3.2), myokinase
(EC 2.7. 4.3), glucose-6-phosphatase (EC 3.1. 3.9), and fructose-1, 6-diphosphatase (EC
3.1. 3.11) reactions have been determined at 38 degrees C, pH 7.0, ionic strength 0.25, and
varying free magnesium concentrations. The equilibrium constant (KCK) for the creatine
kinase reaction defined as: KCK=[sigma ATP][sigma creatine] divided by ([sigma
ADP][sigma creatine-P][H+]) was measured at 0.25 ionic strength and 38 degrees C and …
(EC 2.7. 4.3), glucose-6-phosphatase (EC 3.1. 3.9), and fructose-1, 6-diphosphatase (EC
3.1. 3.11) reactions have been determined at 38 degrees C, pH 7.0, ionic strength 0.25, and
varying free magnesium concentrations. The equilibrium constant (KCK) for the creatine
kinase reaction defined as: KCK=[sigma ATP][sigma creatine] divided by ([sigma
ADP][sigma creatine-P][H+]) was measured at 0.25 ionic strength and 38 degrees C and …
