Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity
CR Wheeler, JA Salzman, NM Elsayed, ST Omaye… - Analytical …, 1990 - Elsevier
CR Wheeler, JA Salzman, NM Elsayed, ST Omaye, DW Korte Jr
Analytical biochemistry, 1990•ElsevierAutomated assays for catalase, glutathione peroxidase, glutathione reductase, and
superoxide dismutase are presented. The assay for catalase is based on the peroxidatic
activity of the enzyme. The glutathione peroxidase and reductase assays measure the
consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized
glutathione, respectively. The assay for superoxide dismutase is based on the reduction of
cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide …
superoxide dismutase are presented. The assay for catalase is based on the peroxidatic
activity of the enzyme. The glutathione peroxidase and reductase assays measure the
consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized
glutathione, respectively. The assay for superoxide dismutase is based on the reduction of
cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide …
Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.
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