In situ hybridization analysis of chick embryos in whole mount and tissue sections
MA Nieto, K Patel, DG Wilkinson - Methods in cell biology, 1996 - Elsevier
Publisher Summary This chapter discusses the in situ hybridization analysis of chick
embryos in whole mount and tissue sections. The in situ analysis of the temporal and spatial
regulation of gene expression in embryos is critical for elucidating the developmental
functions of genes and for unraveling the cellular interactions that regulate tissue patterning
and differentiation. The patterns of gene expression can be visualized, either by detecting
messenger RNA (mRNA) using in situ hybridization or by detecting the encoded protein …
embryos in whole mount and tissue sections. The in situ analysis of the temporal and spatial
regulation of gene expression in embryos is critical for elucidating the developmental
functions of genes and for unraveling the cellular interactions that regulate tissue patterning
and differentiation. The patterns of gene expression can be visualized, either by detecting
messenger RNA (mRNA) using in situ hybridization or by detecting the encoded protein …