Induction and maintenance of differentiation of rat lens epithelium by FGF-2, insulin and IGF-1
EJ Klok, NH Lubsen, CG CHAMBERLAIN… - Experimental eye …, 1998 - Elsevier
The differentiation of rat lens epithelial cells to fibre cells can be mimicked using lens
epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor
(FGF). A previous study demonstrated that FGF is required only for initiation of differentiation:
once induced by FGF, differentiation can be maintained by insulin (as assessed by following
the accumulation of fibre-cell specific crystallins). The aim of this investigation was to
determine whether insulin-like growth factor 1 (IGF-1) can also maintain differentiation and …
epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor
(FGF). A previous study demonstrated that FGF is required only for initiation of differentiation:
once induced by FGF, differentiation can be maintained by insulin (as assessed by following
the accumulation of fibre-cell specific crystallins). The aim of this investigation was to
determine whether insulin-like growth factor 1 (IGF-1) can also maintain differentiation and …