Insulin and IGF-I affect the protein composition of the lens fibre cell with possible consequences for cataract
A Civil, ST van Genesen, EJ Klok, NH Lubsen - Experimental eye research, 2000 - Elsevier
Explanted newborn rat lens epithelial cells were cultured with various concentrations of FGF-
2 and/or insulin or IGF-I for 8–20 days. The accumulation of αA-, αB-, βA3/1-, βB2-and γA-F-
crystallin was measured. During culture with insulin only, ie in the absence of fibre cell
differentiation, αA-and αB-crystallin accumulated to the same level as found in differentiating
cells. Culture of epithelial cells with IGF-I led to an increase in αB-crystallin, but not in αA-
crystallin. The addition of insulin under differentiation conditions (in the presence of 25ng …
2 and/or insulin or IGF-I for 8–20 days. The accumulation of αA-, αB-, βA3/1-, βB2-and γA-F-
crystallin was measured. During culture with insulin only, ie in the absence of fibre cell
differentiation, αA-and αB-crystallin accumulated to the same level as found in differentiating
cells. Culture of epithelial cells with IGF-I led to an increase in αB-crystallin, but not in αA-
crystallin. The addition of insulin under differentiation conditions (in the presence of 25ng …