[HTML][HTML] Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells
SW Cowan-Jacob, H Zhang, M Simonen… - Journal of Biological …, 2000 - ASBMB
BFL-1 is the smallest member of the BCL-2 family and has been shown to retard apoptosis
in various cell lines. However, the structural basis for its function remains unclear. Molecular
modeling showed that BFL-1 could have a similar core structure as BCL-xL, consisting of
seven α helices, although both proteins share only the conserved BCL-2 homology domains
(BH1 and BH2 domains), but otherwise have very limited sequence homology, particularly in
the N-terminal region. We demonstrated in the yeast two-hybrid system that BFL-1 interacts …
in various cell lines. However, the structural basis for its function remains unclear. Molecular
modeling showed that BFL-1 could have a similar core structure as BCL-xL, consisting of
seven α helices, although both proteins share only the conserved BCL-2 homology domains
(BH1 and BH2 domains), but otherwise have very limited sequence homology, particularly in
the N-terminal region. We demonstrated in the yeast two-hybrid system that BFL-1 interacts …
