[HTML][HTML] Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain
A Nohturfft, MS Brown, JL Goldstein - Journal of Biological Chemistry, 1998 - ASBMB
The NH 2-terminal fragments of sterol regulatory element-binding proteins (SREBPs) are
released from endoplasmic reticulum membranes by proteases whose activities depend
upon SREBP cleavage-activating protein (SCAP), a polytopic endoplasmic reticulum
membrane protein. The activity of SCAP is inhibited by sterols, which appear to interact with
the polytopic membrane domain of SCAP. Here, we use protease protection and N-linked
glycosylation site-mapping techniques to define the topology of the eight membrane …
released from endoplasmic reticulum membranes by proteases whose activities depend
upon SREBP cleavage-activating protein (SCAP), a polytopic endoplasmic reticulum
membrane protein. The activity of SCAP is inhibited by sterols, which appear to interact with
the polytopic membrane domain of SCAP. Here, we use protease protection and N-linked
glycosylation site-mapping techniques to define the topology of the eight membrane …
