Intracellular transport of cholesterol to the plasma membrane.
RF DeGrella, RD Simoni - Journal of Biological Chemistry, 1982 - Elsevier
RF DeGrella, RD Simoni
Journal of Biological Chemistry, 1982•ElsevierWe have modified a plasma membrane isolation procedure which utilizes DEAE-Sephadex
beads (Gotlib, LJ, and Searls, DB (1980) Biochim. Biophys. Acta 602, 207-212) to rapidly
measure intracellular transport of cholesterol from the site of synthesis in the endoplasmic
reticulum to the plasma membrane. This transport process is rapid, with a half-time of about
10 min, has different kinetics from that of intracellular glycoprotein transport, and appears to
be energy-dependent.
beads (Gotlib, LJ, and Searls, DB (1980) Biochim. Biophys. Acta 602, 207-212) to rapidly
measure intracellular transport of cholesterol from the site of synthesis in the endoplasmic
reticulum to the plasma membrane. This transport process is rapid, with a half-time of about
10 min, has different kinetics from that of intracellular glycoprotein transport, and appears to
be energy-dependent.
We have modified a plasma membrane isolation procedure which utilizes DEAE-Sephadex beads (Gotlib, L. J., and Searls, D. B. (1980) Biochim. Biophys. Acta 602, 207-212) to rapidly measure intracellular transport of cholesterol from the site of synthesis in the endoplasmic reticulum to the plasma membrane. This transport process is rapid, with a half-time of about 10 min, has different kinetics from that of intracellular glycoprotein transport, and appears to be energy-dependent.
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