[HTML][HTML] Mitochondrial release of pro-apoptotic proteins: electrostatic interactions can hold cytochrome c but not Smac/DIABLO to mitochondrial membranes
RT Uren, G Dewson, C Bonzon, T Lithgow… - Journal of Biological …, 2005 - ASBMB
A key step in the initiation of apoptosis is the release from the mitochondrial intermembrane
space of cytochrome c and other pro-apoptotic proteins such as Smac/DIABLO, Omi/HtrA2,
apoptosis-inducing factor (AIF), and endonuclease G (EndoG). Discrepancies have arisen,
however, as to whether all these proteins are released in different systems. Our results
suggest that failure to observe cytochrome c release may be due to the use of different
buffers because after permeabilization by caspase-8 cleaved human Bid (tBid), cytochrome …
space of cytochrome c and other pro-apoptotic proteins such as Smac/DIABLO, Omi/HtrA2,
apoptosis-inducing factor (AIF), and endonuclease G (EndoG). Discrepancies have arisen,
however, as to whether all these proteins are released in different systems. Our results
suggest that failure to observe cytochrome c release may be due to the use of different
buffers because after permeabilization by caspase-8 cleaved human Bid (tBid), cytochrome …