[HTML][HTML] Molecular cloning, enzymatic characterization, developmental expression, and cellular localization of a mouse cytochrome P450 highly expressed in kidney
J Ma, W Qu, PE Scarborough, KB Tomer… - Journal of Biological …, 1999 - ASBMB
A cDNA encoding a new cytochrome P450 was isolated from a mouse liver library.
Sequence analysis reveals that this 1,886-base pair cDNA encodes a 501-amino acid
polypeptide that is 69–74% identical to CYP2J subfamily P450s and is designated CYP2J5.
Recombinant CYP2J5 was co-expressed with NADPH-cytochrome P450 oxidoreductase in
Sf9 cells using a baculovirus system. Microsomal fractions of CYP2J5/NADPH-cytochrome
P450 oxidoreductase-transfected cells metabolize arachidonic acid to 14, 15-, 11, 12-, and …
Sequence analysis reveals that this 1,886-base pair cDNA encodes a 501-amino acid
polypeptide that is 69–74% identical to CYP2J subfamily P450s and is designated CYP2J5.
Recombinant CYP2J5 was co-expressed with NADPH-cytochrome P450 oxidoreductase in
Sf9 cells using a baculovirus system. Microsomal fractions of CYP2J5/NADPH-cytochrome
P450 oxidoreductase-transfected cells metabolize arachidonic acid to 14, 15-, 11, 12-, and …
