A demineralization procedure for immunohistopathological use: EDTA treatment preserves lymphoid cell surface antigens
R Jonsson, A Tarkowski, L Klareskog - Journal of immunological methods, 1986 - Elsevier
An evaluation of the usefulness of EDTA treatment for decalcification of murine bone tissue
in order to preserve both morphological details and immunologically intact cell surface
antigens has been performed. The ABC immunohistochemical staining technique employing
monoclonal antibodies to subsets of T-lymphocytes, B-lympocytes and to Ia antigens was
used on frozen sections. Treatment of mouse hindlegs with EDTA for 14 days resulted in an
efficient decalcification and good preservation of morphological details. When lymphoid …
in order to preserve both morphological details and immunologically intact cell surface
antigens has been performed. The ABC immunohistochemical staining technique employing
monoclonal antibodies to subsets of T-lymphocytes, B-lympocytes and to Ia antigens was
used on frozen sections. Treatment of mouse hindlegs with EDTA for 14 days resulted in an
efficient decalcification and good preservation of morphological details. When lymphoid …
