Differential dependence of the D1 and D5Dopamine receptors on the G protein γ7 subunit for activation of Adenylylcyclase
Q Wang, JP Jolly, JD Surmeier, BM Mullah… - Journal of Biological …, 2001 - ASBMB
The D 1 dopamine receptor, G protein γ 7 subunit, and adenylylcyclase are selectively
expressed in the striatum, suggesting their potential interaction in a common signaling
pathway. To evaluate this possibility, a ribozyme strategy was used to suppress the
expression of the G protein γ 7 subunit in HEK 293 cells stably expressing the human D 1
dopamine receptor. Prior in vitro analysis revealed that the γ 7 ribozyme possessed
cleavage activity directed exclusively toward the γ 7 RNA transcript (Wang, Q., Mullah, B …
expressed in the striatum, suggesting their potential interaction in a common signaling
pathway. To evaluate this possibility, a ribozyme strategy was used to suppress the
expression of the G protein γ 7 subunit in HEK 293 cells stably expressing the human D 1
dopamine receptor. Prior in vitro analysis revealed that the γ 7 ribozyme possessed
cleavage activity directed exclusively toward the γ 7 RNA transcript (Wang, Q., Mullah, B …
