[HTML][HTML] Combined laser capture microdissection and serial analysis of gene expression from human tissue samples
JH Cho-Vega, P Troncoso, KA Do, C Rago, X Wang… - Modern pathology, 2005 - Elsevier
Cell-specific gene expression profiling from heterogeneous human tissues is confounded by
cell purification limitations. Here, we describe a technique to generate gene expression
profiles of pure populations of prostate cancer cells obtained from fresh-frozen
prostatectomy specimens and small initial quantities of RNA by combining laser capture
microdissection and microserial analysis of gene expression (LCM–microSAGE). Two
microSAGE libraries were obtained from approximately 100 000 laser pulses, estimated to …
cell purification limitations. Here, we describe a technique to generate gene expression
profiles of pure populations of prostate cancer cells obtained from fresh-frozen
prostatectomy specimens and small initial quantities of RNA by combining laser capture
microdissection and microserial analysis of gene expression (LCM–microSAGE). Two
microSAGE libraries were obtained from approximately 100 000 laser pulses, estimated to …
