Microarray‐based genotyping for blood groups: comparison of gene array and 5′‐nuclease assay techniques with human platelet antigen as a model
P Bugert, S McBride, G Smith, A Dugrillon… - …, 2005 - Wiley Online Library
P Bugert, S McBride, G Smith, A Dugrillon, H Klüter, WH Ouwehand, P Metcalfe
Transfusion, 2005•Wiley Online LibraryBACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs)
are based on single‐nucleotide polymorphisms (SNPs) in genes encoding relevant
membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen
(HPA) systems as a model, the suitability of a fourth‐generation microarray technique for
SNP typing was investigated. The results of the former were compared with those of a
parallel developed third‐generation technique (TaqMan assay, Applied Biosystems). Both …
are based on single‐nucleotide polymorphisms (SNPs) in genes encoding relevant
membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen
(HPA) systems as a model, the suitability of a fourth‐generation microarray technique for
SNP typing was investigated. The results of the former were compared with those of a
parallel developed third‐generation technique (TaqMan assay, Applied Biosystems). Both …
BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single‐nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins.
STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth‐generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third‐generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA‐1, ‐2, ‐3, ‐5, and‐15 systems.
RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples.
CONCLUSION: The data presented here validate the use of microarray for large‐scale SNP typing for clinically relevant blood group alloantigens.
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