Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.
J Laval, J Pierre, F Laval - Proceedings of the National …, 1981 - National Acad Sciences
J Laval, J Pierre, F Laval
Proceedings of the National Academy of Sciences, 1981•National Acad SciencesCell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-
methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of
7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not
liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are
different. The release of 7-methylguanine by chemical depurination of ethanol-soluble
oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also …
methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of
7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not
liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are
different. The release of 7-methylguanine by chemical depurination of ethanol-soluble
oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also …
Cell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are different. The release of 7-methylguanine by chemical depurination of ethanol-soluble oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also found in Escherichia coli.
National Acad Sciences
