A new screening test for C3 nephritis factor based on a stable cell bound convertase on sheep erythrocytes
U Rother - Journal of immunological methods, 1982 - Elsevier
C3 nephritis factor (C3nef) activity was measured by the incubation of sheep erythrocytes
with a mixture of normal human serum and patient's serum in EGTA followed by lysis of the
washed cells with rat C in EDTA. The C convertase activity on the cells was dependent on
the dose of patient's serum. Activation of human C3–C9 by the washed cells was shown by
lysis of susceptible target cells (chicken erythrocytes). The test is specific for C3nef.
with a mixture of normal human serum and patient's serum in EGTA followed by lysis of the
washed cells with rat C in EDTA. The C convertase activity on the cells was dependent on
the dose of patient's serum. Activation of human C3–C9 by the washed cells was shown by
lysis of susceptible target cells (chicken erythrocytes). The test is specific for C3nef.
