HPLC simultaneous determination of glycerol and mannitol in human tissues for forensic analysis
A Kiyoshima, K Kudo, N Nishida, N Ikeda - Forensic science international, 2002 - Elsevier
A method for simultaneous determination of glycerol and mannitol in various human tissues
was devised and for this we used high-performance liquid chromatography (HPLC).
Specimens were homogenized in a mixture of chloroform and methanol, phosphate buffer
(pH 7.0) and pentaerythritol (IS) solution. After centrifugation, an aliquot of the aqueous layer
was evaporated to dryness and derivatized with p-nitrobenzoyl chloride at 50° C for 1h, then
applied to HPLC with analytical conditions of: column, CAPCELL PAK C18 MG (250 mm× …
was devised and for this we used high-performance liquid chromatography (HPLC).
Specimens were homogenized in a mixture of chloroform and methanol, phosphate buffer
(pH 7.0) and pentaerythritol (IS) solution. After centrifugation, an aliquot of the aqueous layer
was evaporated to dryness and derivatized with p-nitrobenzoyl chloride at 50° C for 1h, then
applied to HPLC with analytical conditions of: column, CAPCELL PAK C18 MG (250 mm× …
