Optimization of methods to study pulmonary dendritic cell migration reveals distinct capacities of DC subsets to acquire soluble versus particulate antigen
Dendritic cell migration from the airway to lymph nodes is a key event in the development of
airway immunity during infection, allergy, and vaccination. To identify the best approaches to
investigate DC migration to lung-draining lymph nodes, we directly compared three methods
previously used to track DC migration: airway administration of fluorescent OVA, latex
beads, or carboxyfluorescein succinimidyl ester (CFSE). We show that two of the methods
employed in optimal conditions—administration of fluorescent OVA or latex particles—have …
airway immunity during infection, allergy, and vaccination. To identify the best approaches to
investigate DC migration to lung-draining lymph nodes, we directly compared three methods
previously used to track DC migration: airway administration of fluorescent OVA, latex
beads, or carboxyfluorescein succinimidyl ester (CFSE). We show that two of the methods
employed in optimal conditions—administration of fluorescent OVA or latex particles—have …