[HTML][HTML] Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin
Background Histone methylation is regulated by a large number of histone
methyltransferases and demethylases. The recently discovered SMCX/KMD5C demethylase
has been shown to remove methyl residues from lysine 4 of histone H3 (H3K4), and
constitutes an important component of the regulatory element-1-silencing transcription factor
(REST) protein complex. However, little is known about the cellular mechanisms that control
SMCX activity and intracellular trafficking. Results In this study, we found that small …
methyltransferases and demethylases. The recently discovered SMCX/KMD5C demethylase
has been shown to remove methyl residues from lysine 4 of histone H3 (H3K4), and
constitutes an important component of the regulatory element-1-silencing transcription factor
(REST) protein complex. However, little is known about the cellular mechanisms that control
SMCX activity and intracellular trafficking. Results In this study, we found that small …
Background
Histone methylation is regulated by a large number of histone methyltransferases and demethylases. The recently discovered SMCX/KMD5C demethylase has been shown to remove methyl residues from lysine 4 of histone H3 (H3K4), and constitutes an important component of the regulatory element-1-silencing transcription factor (REST) protein complex. However, little is known about the cellular mechanisms that control SMCX activity and intracellular trafficking.
Results
In this study, we found that small interfering RNA-mediated knockdown of proliferating cell nuclear antigen (PCNA) resulted in the reduction of the chromatin-bound SMCX fraction. We identified a PCNA-interaction protein motif (PIP box) in the SMCX protein. Using site-directed mutagenesis, we found that the amino acids of the SMCX PIP box are involved in the association of SMCX with PCNA and its interaction with chromatin.
Conclusions
Our data indicate that the intracellular trafficking of SMCX is controlled by its association with PCNA.
Springer