Sequence, genomic structure, and chromosomal assignment of human DOC-2
HM Albertsen, SA Smith, R Melis, B Williams, P Holik… - Genomics, 1996 - Elsevier
HM Albertsen, SA Smith, R Melis, B Williams, P Holik, J Stevens, R White
Genomics, 1996•ElsevierDOC-2 is a human gene originally identified as a 767-bp cDNA fragment isolated from
normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We
have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and
localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5′-end of DOC-2
has also been sequenced, revealing the intron–exon structure of the first eight exons (788
bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of …
normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We
have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and
localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5′-end of DOC-2
has also been sequenced, revealing the intron–exon structure of the first eight exons (788
bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of …
DOC-2 is a human gene originally identified as a 767-bp cDNA fragment isolated from normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5′-end of DOC-2 has also been sequenced, revealing the intron–exon structure of the first eight exons (788 bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of 770 amino acid residues with a molecular weight of 82.5 kDa. Comparison of the DNA and amino acid sequences of DOC-2 to publicly accessible sequence databases revealed 83% identity to p96, a murine protein of similar size, thought to be a mitogen-responsive phosphoprotein. In addition, about 45% identity was observed between the first 140 N-terminal residues of DOC-2 and theCaenorhabditas elegansM110.5 andDrosophila melanogasterDab genes.
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