[HTML][HTML] Argininosuccinate synthetase overexpression in vascular smooth muscle cells potentiates immunostimulant-induced NO production
L Xie, SS Gross - Journal of Biological Chemistry, 1997 - ASBMB
Immunostimulants trigger vascular smooth muscle cells (VSMC) to express both the
inducible isoform of NO synthase (iNOS) and argininosuccinate synthetase (AS). With
constitutively expressed argininosuccinate lyase (AL), AS confers cells with an Arg/Cit cycle
that can sustain NO production via continuous regeneration of the NOS substrate, l-arginine
(Arg), from the NOS coproduct, l-citrulline (Cit). To assess whether NO synthesis can be rate-
limited by Arg recycling, we tested whether AS-overexpressing cells have an enhanced …
inducible isoform of NO synthase (iNOS) and argininosuccinate synthetase (AS). With
constitutively expressed argininosuccinate lyase (AL), AS confers cells with an Arg/Cit cycle
that can sustain NO production via continuous regeneration of the NOS substrate, l-arginine
(Arg), from the NOS coproduct, l-citrulline (Cit). To assess whether NO synthesis can be rate-
limited by Arg recycling, we tested whether AS-overexpressing cells have an enhanced …