Effect of chronic hyperglycemia on in vivo insulin secretion in partially pancreatectomized rats.

L Rossetti, GI Shulman, W Zawalich… - The Journal of …, 1987 - Am Soc Clin Investig
L Rossetti, GI Shulman, W Zawalich, RA DeFronzo
The Journal of clinical investigation, 1987Am Soc Clin Investig
We have examined the effect of chronic (4 wk) hyperglycemia on insulin secretion in vivo in
an awake, unstressed rat model. Three groups of animals were examined: control, partial
(90%) pancreatectomy, and partial pancreatectomy plus phlorizin, in order to normalize
plasma glucose levels. Insulin secretion in response to arginine (2 mM), hyperglycemia (+
100 mg/dl), and arginine plus hyperglycemia was evaluated. In diabetic compared with
control animals three specific alterations were observed:(a) a deficient insulin response, in …
We have examined the effect of chronic (4 wk) hyperglycemia on insulin secretion in vivo in an awake, unstressed rat model. Three groups of animals were examined: control, partial (90%) pancreatectomy, and partial pancreatectomy plus phlorizin, in order to normalize plasma glucose levels. Insulin secretion in response to arginine (2 mM), hyperglycemia (+100 mg/dl), and arginine plus hyperglycemia was evaluated. In diabetic compared with control animals three specific alterations were observed: (a) a deficient insulin response, in both first and second phases, to hyperglycemia; (b) an augmented insulin response to the potentiating effect of arginine under basal glycemic conditions; and (c) an inability of hyperglycemia to augment the potentiating effect of arginine above that observed under basal glycemic conditions. Normalization of the plasma glucose profile by phlorizin treatment in diabetic rats completely corrected all three beta cell abnormalities. These results indicate that chronic hyperglycemia can lead to a defect in in vivo insulin secretion which is reversible when normoglycemia is restored.
The Journal of Clinical Investigation