Function of GATA transcription factors in preadipocyte-adipocyte transition

Q Tong, G Dalgin, H Xu, CN Ting, JM Leiden… - Science, 2000 - science.org
Q Tong, G Dalgin, H Xu, CN Ting, JM Leiden, GS Hotamisligil
Science, 2000science.org
Genes that control the early stages of adipogenesis remain largely unknown. Here, we show
that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors
and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2
and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the
preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of
peroxisome proliferator–activated receptor γ. GATA-3–deficient embryonic stem cells exhibit …
Genes that control the early stages of adipogenesis remain largely unknown. Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of peroxisome proliferator–activated receptor γ. GATA-3–deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition.
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