Adenovirus‐mediated artificial microRNA against human fibrinogen like protein 2 inhibits hepatocellular carcinoma growth
M Wang, J Liu, D Xi, X Luo… - The journal of gene …, 2016 - Wiley Online Library
M Wang, J Liu, D Xi, X Luo, Q Ning
The journal of gene medicine, 2016•Wiley Online LibraryBackground Human fibrinogen‐like protein 2 (hFGL2) is overexpressed in various human
solid tumors and is associated with tumor growth, invasion and angiogenesis of
hepatocellular carcinoma (HCC) LM6 cells. In the present study, an adenoviral vector
expressing an artificial microRNA (miRNA) against hfgl2 (Ad‐hfgl2‐miRNA) was used to
evaluate the effect of FGL2 knockdown on cell proliferation and tumor growth of HCCLM6
xenografts. Methods Ad‐hfgl2‐miRNA was constructed and its effect on hFGL2 expression …
solid tumors and is associated with tumor growth, invasion and angiogenesis of
hepatocellular carcinoma (HCC) LM6 cells. In the present study, an adenoviral vector
expressing an artificial microRNA (miRNA) against hfgl2 (Ad‐hfgl2‐miRNA) was used to
evaluate the effect of FGL2 knockdown on cell proliferation and tumor growth of HCCLM6
xenografts. Methods Ad‐hfgl2‐miRNA was constructed and its effect on hFGL2 expression …
Background
Human fibrinogen‐like protein 2 (hFGL2) is overexpressed in various human solid tumors and is associated with tumor growth, invasion and angiogenesis of hepatocellular carcinoma (HCC) LM6 cells. In the present study, an adenoviral vector expressing an artificial microRNA (miRNA) against hfgl2 (Ad‐hfgl2‐miRNA) was used to evaluate the effect of FGL2 knockdown on cell proliferation and tumor growth of HCCLM6 xenografts.
Methods
Ad‐hfgl2‐miRNA was constructed and its effect on hFGL2 expression and cell proliferation was analyzed. The xenograft tumor model was established in nude mice and this was accompanied by intratumoral injection of Ad‐hfgl2‐miRNA when the tumor volume had reached 100 mm3. The volume of the tumor xenograft was recorded and angiogenesis in the tumor xenograft was assessed.
Results
Ad‐hfgl2‐miRNA significantly downregulated hFGL2 expression at both the mRNA and protein levels and exerted an inhibitory effect on proliferation in HCCLM6 cells. In HCCLM6 tumor‐bearing mice, the tumor volume significantly decreased following the intratumoral injection of Ad‐hfgl2‐miRNA compared to that of control groups. Additionally, Ad‐hfgl2‐miRNA also exhibited potent anti‐angiogenic effects.
Conclusions
HFGL2 might be a potential target for controlling angiogenesis in HCC and hfgl2 gene silencing via miRNA may be an alternative strategy for the management of HCC. Copyright © 2016 John Wiley & Sons, Ltd.
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