Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high-content analysis
AM Smith, HM Gibbons, C Lill, RLM Faull… - Microglia: Methods and …, 2013 - Springer
AM Smith, HM Gibbons, C Lill, RLM Faull, M Dragunow
Microglia: Methods and Protocols, 2013•SpringerMicroglia are thought to be involved in diseases of the adult human brain as well as normal
aging processes. While neonatal and rodent microglia are often used in studies
investigating microglial function, there are important differences between rodent microglia
and their adult human counterparts. Human brain tissue provides a unique and valuable tool
for microglial cell and molecular biology. Routine protocols can now enable use of this
culture method in many laboratories. Detailed protocols and advice for culture of human …
aging processes. While neonatal and rodent microglia are often used in studies
investigating microglial function, there are important differences between rodent microglia
and their adult human counterparts. Human brain tissue provides a unique and valuable tool
for microglial cell and molecular biology. Routine protocols can now enable use of this
culture method in many laboratories. Detailed protocols and advice for culture of human …
Abstract
Microglia are thought to be involved in diseases of the adult human brain as well as normal aging processes. While neonatal and rodent microglia are often used in studies investigating microglial function, there are important differences between rodent microglia and their adult human counterparts. Human brain tissue provides a unique and valuable tool for microglial cell and molecular biology. Routine protocols can now enable use of this culture method in many laboratories. Detailed protocols and advice for culture of human brain microglia are provided here. We demonstrate the protocol for culturing human adult microglia within a mixed glial culture and use a phagocytosis assay as an example of the functional studies possible with these cells as well as a high-content analysis method of quantification.
Springer
