Targeted single-cell RNA-seq identifies minority cell types of kidney distal nephron
L Chen, CL Chou, MA Knepper - Journal of the American Society …, 2021 - journals.lww.com
Background Proximal tubule cells dominate the kidney parenchyma numerically, although
less abundant cell types of the distal nephron have disproportionate roles in water and
electrolyte balance. Methods Coupling of a FACS-based enrichment protocol with single-cell
RNA-seq profiled the transcriptomes of 9099 cells from the thick ascending limb
(CTAL)/distal convoluted tubule (DCT) region of the mouse nephron. Results Unsupervised
clustering revealed Slc12a3+/Pvalb+ and Slc12a3+/Pvalb− cells, identified as DCT1 and …
less abundant cell types of the distal nephron have disproportionate roles in water and
electrolyte balance. Methods Coupling of a FACS-based enrichment protocol with single-cell
RNA-seq profiled the transcriptomes of 9099 cells from the thick ascending limb
(CTAL)/distal convoluted tubule (DCT) region of the mouse nephron. Results Unsupervised
clustering revealed Slc12a3+/Pvalb+ and Slc12a3+/Pvalb− cells, identified as DCT1 and …