[HTML][HTML] Palmitate stimulates the epithelial sodium channel by elevating intracellular calcium, reactive oxygen species, and phosphoinositide 3-kinase activity
QS Wang, C Liang, N Niu, X Yang, X Chen… - … Medicine and Cellular …, 2018 - hindawi.com
Previous studies indicate that the epithelial sodium channel (ENaC) in the kidney is
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca 2+ and that after
chelating intracellular Ca 2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP 3 …
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca 2+ and that after
chelating intracellular Ca 2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP 3 …
[PDF][PDF] Research Article Palmitate Stimulates the Epithelial Sodium Channel by Elevating Intracellular Calcium, Reactive Oxygen Species, and Phosphoinositide 3 …
QS Wang, C Liang, N Niu, X Yang, X Chen, BL Song… - 2018 - academia.edu
Previous studies indicate that the epithelial sodium channel (ENaC) in the kidney is
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP3 …
upregulated in diabetes mellitus. Here, we show that ENaC single-channel activity in distal
nephron cells was significantly increased by palmitate, a free fatty acid which is elevated in
diabetes mellitus. We also show that palmitate increased intracellular Ca2+ and that after
chelating intracellular Ca2+ with BAPTA-AM, palmitate failed to affect ENaC activity.
Treatment of the cells with 2-aminoethoxydiphenyl borate (2-APB, an inhibitor of IP3 …